Using a calcium assay is the process of determining the amount of calcium in a sample. Calcium assays are used in a variety of areas, including biochemistry, medical research, and industrial applications. Below is more information regarding the uses and indicators of calcium assay research projects.

Synthetic Calcium Indicators

Several types of synthetic calcium indicators have been developed in recent years for calcium assays. Currently, these indicators are the most common probes in high-throughput calcium assays. However, they have limitations. In order to determine whether synthetic calcium indicators can meet your needs, it is important to compare them to each other.

A key benefit of these indicators is their high affinity to calcium ions. This allows them to detect single 250 nM calcium peaks. In addition, they can be used to tag specific proteins. They also have the potential to be manipulated using standard molecular biology techniques.

Activation Of TRPV1

Activation of the endoplasmic reticulum ryannodine receptor (ERR) TRPV1 results in a large steady-state current in a calcium assay. We investigated the effect of TRPV1 on the steady-state current-voltage relationship in transfected HEK293 cells. We observed that TRPV1 selective agonist CAP (capsaicin) and the non-selective agonist AEA (anandamide) elicited identical whole-cell current traces in the presence of calcium. However, the CAP-induced currents were saturating, while the AEA-induced currents were smaller.

Activation Of GluA1o-g4 Receptors

Activation of GluA1o-g4 receptors by calcium assay has been studied in model organisms and in humans. The ability to monitor calcium levels in cells is a valuable technique for modern drug discovery and can also help to predict unwanted drug side effects. Moreover, calcium signalling can be studied to better understand physiological processes and pathogenesis of diseases. In the study described herein, several assays are described to provide in situ information about signalling events. These assays are designed to avoid sources of error and provide easy-to-quantify results.

Calcium Flux Assays

Detecting intracellular calcium mobilization in cells is important for many high-throughput assays used in drug discovery. Calcium is an essential cellular second messenger that modulates a variety of cellular processes. It is used by hundreds of proteins to regulate intracellular calcium levels and to signal cellular functions.

Calcium assays can be conducted with fluorescent dyes or synthetic indicators. Calcium flux assays are one of the most commonly used assay technologies. Unlike most assays, which depend on preloading or adding an inhibitor, calcium flux assays detect the intracellular calcium mobilization after the release of Ca2+ into the cytoplasm.